Abstract

All of the methods for determining the level of rabies antibodies used rabies viruses as antigen. Attenuated viruses can revert to a more virulent form, and inactivated virus may produce serious side effects. Anti-idiotype Ab can be used as surrogate antigen in serological test. The aim of this study was to prepare the anti-idiotype antibody as diagnostic kit in rabies serological test. Polyclonal anti-idiotype Ab were prepared in laying chickens. Rabbit anti dog immunoglobuline were prepared used New Zeeland White strain, and dog anti rabies serum were prepared from rabies immunized dog. The stages are making of stocks A, stocks B and prototype diagnostic kit. Stocks A is a mixture of S. aureus Cowan I intact in solution of Tris Buffer HCl with rabbit anti dog serum in various comparisons (v/v). Stocks B is a mixture of rabies anti-idiotype Ab with rabies antibody. Comparison of the obtained between whole S. aureus Cowan I and rabbit serum anti dog is 4-6:2-4. Optimization of stocks A and B was needed and will be a candidate when the diagnostic kits on the positive control produces coagglutination and negative controls do not produce coagglutination.

Keywords: antibody, anti-idiotype antibodi, rabies, kit diagnostic, serological test