Abstract

In this study, the separation of active extracts from Tengkawang seed oil (Shorea sumatrana Sym.) has successfully carried out. The extract was obtained by fractionation of the most toxic fraction of Artemia salina Leach. with LC50 2.55 x 10 ppm, using Brine Shrimp Lethality Test (BSLT), and is antibacterial against Staphylococcus aureus with the value of Maximum Inhibition Concentration (MIC) is 5%, and 14.41 mm diameter of inhibition with Method Wells Plate.

Keywords: Shorea sumatrana Sym, A. salina Leach., Staphylococcus aureus.