Abstract:

Nampu (Homalomena rosrata Griff.) is traditionally used to increase sexual activity for men. However, the lack of scientific information on the phytochemical content of Nampu plants needs further research on the secondary metabolite compounds for isolation and identification purposes. The phytochemical screening of raw material, ethanol extract, and water fraction show positive results for flavonoid, tannin, polyphenol, quinone, monoterpenoid-sesquiterpenoid, and steroid-triterpenoid groups. The chloroform fractions were monitored by the thin-layer chromatography (TLC) method with the addition of KOH 5%. The results showed a blue spot under UV light at 365 nm after the addition of KOH 5%. The fraction was purified by a preparative thin-layer chromatography method (TLC-P), while the isolates' purity was tested by the two-dimensional TLC method and TLC with three different phases of mobile phases. The results of the analysis with the spectrophotometer UV-Visible showed a wavelength of 278.8 nm and 213.2 nm. In addition, the functional group analysis using the I.R. spectrometer showed O.H. stretch at wave numbers 3419.79 cm1,strain C = C aromatic at the wave number 1585.49 cm , aromatic C-H at wave number 2922.16 cm-1, C = O at the wave number 1672.28 cm -1. Based on TLC and spectrophotometric data, the isolates were coumarin compounds.

Keywords: Nampu (Homalomena rosrata Griff.); isolation; thin layer chromatography;spectrophotometer UV-Visible; spectroscopy infrared; coumarin