This research aims to obtain antibacterial compound from leaves of Hydrangea macrophylla. The isolation
stage started with the extraction by using n-hexane and methanol. Then the methanol extract was separated by
Vacuum Liquid Chromatography (VLC) method and was purified by Radial Chromatography which was
guided by Thin Layer Chromatography (TLC) method. Two secondary metabolites were isolated i.e. (-)-
hydrangenol and thunberginol A. Their chemical structures have been determined on the basis of
chemical and physicochemical evidence. The antibacterial assay was performed by
microdilution method against bacteria pathogenic: Salmonella thypi, Shigella dysenteriae, Escherichia
coli, Staphylococcus aureus, Pseudomonas aureginosa and Klebsiella pneumoniae. On antibacterial test both
compound were found as mostly weak antibacterials.
Keywords: Hydrangea macrophylla, (-)-hydrangenol, thunberginol A, antibacterial activity.